Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
نویسندگان
چکیده
منابع مشابه
CRISPR/Cas9 in Genome Editing and Beyond.
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has ...
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Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the ...
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The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5'-NGG-3' protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9), and its KKH variant, successfully induced targeted mutagenesis with high frequency in...
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This article reviews ethical issues related to genome editing using CRISPR/Cas9 system. The use of CRISPR/ cas9 revives many previous social and ethical issues with humans, other organisms and the environment, such as taking into account the non-maleficence principle in risk assessment, genome editing in germline, safety issues to avoid ecological impairment or the possible use of the technique...
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The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we brie...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2018
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2765